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[9000493.] رقم البحث : 9000493 - |
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Detection of pathogenic bacteria in milk and its products / |
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Agricultural Research Journal Suez Canal University / |
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Hassan A.M.Al-Demerdash |
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Mutlag M. Al-Otaibi |
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pathogenic bacteria; detection; PCR; dairy products |
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The detection of major pathogenic bacteria is an important measurement for providing health guarantee for milk and milk-related products. So, this research was directed to develop more simple, rapid and sensitive detection method in the field of food hygiene by using the universal PCR. In this work three common pathogenic bacteria were detected and differentiated named (Staphylococcus aureus, Salmonella and Escherichia coli) in 120 suspect isolates from milk and dairy products collected from farmer vendors and dairy shops in Al-Ahsa area. A primer targeting of the thermostable nuclease gene (nuc of Staphlococcus, hilA genes of Salmonella and A SLT genes of E.coli) were used in the PCR analysis. A DNA fragment of 279, 972 and 660 bp was amplified for Staphylococcus aureus, Salmonella and Escherichia coli, respectively. Result, showed that, the PCR method owned a higher detection rate than others. The detection rate of positive samples by using PCR was 51.6, 12.5 and 93.3 % for Staphylococcus aureus, Salmonella and Escherichia coli, respectively.
By using this technique a rapid, sensitive, and effective results for PCR detection of pathogenic bacteria (both live and dead) in milk and milk products. |
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