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Abstract
In order to improve insect resistance in canola,transgenic canola plants expressing the cry1Ia 5 gene were developed. The cultivars tested differ genetically for callus induction and somatic embryo formation. The cultivar serw - 4 exhibited high regeneration efficiency (97%) followed by serw - 3 (88%). The genetic differences in regeneration ability among the four canola cultivars were resolved by RAPD and AFLP analyses. The genotype - specific markers were determined which can be verified as useful genetic markers for the regeneration capacity of the different canola cultivar used. The efficiency of sonication assisted agrobacterium and particle bombardment mediated gene delivery systems was compared. The data indicate that 5 sec. Sonication followed by agrobacterium infection improves the transformation efficiency to 55% and 33% compared with 25% and 16% that resulted from biolistic gun for the cultivars serw - 4 and serw - 3, respectively. The number of transgenic plants recovered from the agro - sonication method was higher in comparison to biolistic mediated transformation. The agro - sonication method was employed to develop transgenic canola plants expressing the cry1Ia 5 gene. Stable integration of the cry1Ia5 gene into plant genome was confirmed by PCR and Southern blot analyses