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العنوان
Molecular characterization of alpha subunit of sodium pump gene and its relation to energy metabolism in Camelus dromedarius /
المؤلف
El-Kassas, Ahmad Hamdy Kamal.
هيئة الاعداد
باحث / أحمد حمدى كمال القصاص
مشرف / محمدعلى
مشرف / مروة ابراهيم عبد الحميد
مشرف / أحمد وردة
مشرف / يوسف عادل بشارة
الموضوع
Energy metabolism.
تاريخ النشر
2022.
عدد الصفحات
55 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2022
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Biochemistry
الفهرس
Only 14 pages are availabe for public view

from 67

from 67

Abstract

Camelus dromedarius is uniquely adapted to survive the desert environment. It has an excellent
ability to compete with dehydration with significant tolerance. Therefore, it offers an excellent
model for studying osmoregulation. Molecular characterization of Na+/K+ ATPase as an
integral regulator of electrolyte normohemostasis affords a better understanding of this
mechanism in camel. Here is the first report to resolve the full-length sequence of alpha-1
subunit of the sodium pump (ATP1A1) gene in the dromedary camel. The recovered full cDNA
of ATP1A1 was submitted to the GenBank (NCBI GenBank accession #MW628635) and
bioinformatically analyzed. The cDNA sequence revealed 3760 bp with an open reading frame
(ORF) of 3066bp encoding a putative 1021 amino acids polypeptide with a molecular mass of
112696Da. Moreover, different organs’ transcriptomic screening was performed by real-time
PCR. The results confirmed a direct function between the ATP1A1 gene expression and the
vital performance of the target organs. The transcript levels of ATP1A1 showed marked upregulation in both the adrenal gland and brain compared to the other studied organs and so
reflecting the role played by the Na+/K+ ATPase in both organs.