الفهرس 
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Abstract
Squamous cell carcinoma (SCC) is the most frequent malignant neoplasm affecting structures of the head and neck. The incidence of SCC has been gradually increasing over the past three decades.
Carcinogenesis was strongly related to the decrease of apoptosis resulting in more cellular proliferation. Dysregulation of cell cycle also playsan important role regarding tumor formation.
This study, aimed to evaluate the effect of culturing AD-MSCs on HEp-2 cell line and to evaluate the expression of p16 tumor suppression gene and Livin one of the IAPs family. Also cell viability and proliferation was recorded.
The study included three major groups, the first one was the HEp2 cell line alone. The second one was HEp2 cell line cultured with the medium of AD-MSCs. The third group was the HEp2 cell line co-cultured with the media of both AD-MSCs and HEp2. Each group was cultured for 24, 48 and 72 hours and the expression of p16 and Livin genes were recorded and evaluated in each time interval. Cell viability was also evaluated in the three major groups within the three durations.
P16 and Livin genes expression were recorded using PCR, while cell viability was evaluated using MTT essay.
The results of this work showed that culturing HEp2 cell line with AD-MSCs medium for 48 hours increased the expression of p16 gene and reduced the expression of Livin when compared to their expression in the HEp2 group alone. In addition, cell proliferation of HEp2 was obviously decreased. Further increase in expression in p16 and more reduction in Livin expression was recorded in the third group when it was cultured for 72 hours.
According to the previously mentioned results, it was concluded that culturing HEp2 with the media of both AD-MSCs and HEp2 for 72 hours, was the best regarding decrease in both genes and proliferation, highlighting the effects of MSCs mediators on decreasing cellular proliferation.
Mesenchymal stem cells could have a strong therapeutic use in the field of cancer.