الفهرس 
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Abstract
Indomethacin is a non-steroidal anti-inflammatory drug, used in the treatment of many inflammatory diseases. The major side effect limiting its clinical use, is the gastric damages.
The aim of this work Was to study the possible protective effects of vitamin c and ranitidine on the gastric lesion induced by indomethacin in adult albino rats.
Materials and methods: Forty adult albino rats are devided into five equal groups: The control group, indomethacin group, indomethacin plus vitamin c, indomethacin plus ranitidine, and indomethacin plus both vitamin c and ranitidine. In the control group, the rats were received normal daily diet for 10 days. In the indomethacin group, the rats were treated with indomethacin at a dose of 25 mg/ kg.B.W by a gastric tube once daily for 10 cosecutivedays. In indomethacin plus vitamin c group, the rats were treated with indomethacin as the second group,in addition to vitamin c at a dose of 400mg/ kg.B.W orally,5 minutes prior to the indomethacin for 10 days. In the fourth group, the rats were treated with indomethacin as the second group, then received ranitidine at a dose of 25 mg/ kg.B.W. orally,5 min prior to the indomethacin, for 10 cosecutive days. The last group was received the indomethacin as the second group, then received both vitamin c and ranitidine as the previous doses,5 min prior to the indomethacin, daily for 10 days. After 10 days from the treatment, all the rats were anaesthetized, and the specimens of the stomach were prepared for light and electron microscopies.
Results: Indomethacin produced erosion and ulceration of the gastric mucosa. There is focal areas of degeneration and infilammatory cell infilteration in the lamina propria, the gastric gland cells showed vacuolation, degeneration and disappearance of their nuclei. It also induced dilatation and congesion of the blood capillaries in the submucose of the stomach, also the surface mucin layer decreased in the thickness. Electron microscopic examination showed that: Indomethacin induced degeneration and vacuolation of the gastric gland cells, with degeneration of their mitochondria and nuclei. Chief cells showed abnormalities in their zymogenic granules which appeared with dark central part and pale periphery. Administration of vitamin c and ranitidine prior to the indomethacin intake, reduced the pathogenic changes of the gastric wall, with slight return to the picture of the control group.
Conclusion: The toxic effects of the indomethacin on the stomach, was minimized by administration of vitamin c or ranitidine or both.