الفهرس | Only 14 pages are availabe for public view |
Abstract The current in vitro study was designed to characterize and evaluate the antibacterial efficiency of calcium hydroxide (Ca (OH)2)loaded on Gum Arabic (GA) nanocarrier against E. faecalis biofilm and to compare this efficiency with conventional Ca (OH)2, GA, Ca (OH)2 NPs, and GA NPs. Material& Methods The nano particle formulations for the tested medications were prepared and characterized using Transmission Electron Microscope (TEM), and Fouriertransform infrared Spectroscopy (FTIR). A total of 135 human mandibular premolars were selected and, their root canals were prepared. Fifteen roots were then sectioned to obtain 30 teeth slices. All prepared root canals (n=120) and teeth slices (n=30) were divided into six groups according to the intracanal medication used. Each group was further subdivided into three subgroups according to evaluation method: CFU test (n=10), Q-PCR test (n=10), and SEM evaluation (n=5). E. faecalis was inoculated in the samples for 21 days to form biofilms then, the corresponding medications were applied for 7 days. The remaining E. faecalis bacteria after medications placement was evaluated. Results: The synthesis of all NPs was confirmed using TEM. The results of the FTIR confirmed that the Ca (OH)2 was successfully loaded on the GA NPs. Also, FTIR confirmed characterization of all NPs through observation of functional groups involved in stabilization of synthesized NPs. Summary 75 Colony forming unit (CFU) assessment: The six studied groups showed a highly significantly difference in CFUs as revealed by ANOVA (p<0.001***). the lowest significant total bacterial count was recorded in group of Ca (OH)2 loaded on GA NPs form (2.20 ± 0.22 CFU/ml). Accordingly, the highest significant total bacterial count was recorded in control group (8.70 ± 0.22 CFU/ml). All groups showed significant difference between each other except Ca (OH)2 and GA groups, also nano Ca (OH)2 and nano GA groups showed no significant difference as revealed by Tukey’s HSD. Quantitative Real-time polymerase chain reaction (Q-PCR) assessment: The six studied groups showed a highly significantly difference in CT values as revealed by ANOVA (p<0.001***). Accordingly, the lowest significant CT was recorded in control group (17.12 ± 0.61). The highest significant CT level was recorded in Ca (OH)2 loaded on GA NPs group (31.80 ± 0.64). All groups showed statistically significant difference between each other as revealed by Tukey’s HSD. Scanning Electron Microscope (SEM) evaluation: SEM showed that biofilm of E. faecalis was diminished in all treatment groups. Nano medications (nano Ca (OH)2, nano GA) showed better bacterial reduction against E. faecalis than conventional ones (Ca (OH)2, GA). Ca (OH)2 loaded on GA NPs showed the greatest bacterial reduction than other groups. |