الفهرس | Only 14 pages are availabe for public view |
Abstract Biological treatment has shown as the most cost effective and eco-friendly technique for removal of pollutant dyes. Synthetic dyes are widely used in the textile industries. When it is released into the environment become dangerous recalcitrant pollutants. In the present study, the physical chemical properties were studied for four different collected wastewater textile samples. Different bacterial isolates were studied for their abilities to decolorize the congo red dye. Incubation time, incubation temperature, pH and the dye concentration were studied as factors affecting the bio-decolorization. The bacterial strains that showed high percentage of decolorization were isolated, purified and identified using 16S rRNA sequence analysis. The biodegradation by-products were analyzed by LC-MS techniques. By using factor statistical design, the optimum conditions which resulted in the high percentage of decolorization were 25 ppm, 35°C, 7 and 4 days for the dye concentration, incubation temperature, pH of the solution and incubation time. Four different bacterial isolates were identified by 16S rRNA sequence analysis as (Bacillus sp. H77, Staphylococcus hominis H77, Alcaligenes faecalis H77, Exiguobacterium sp. H77). The LC-MS confirmed congo red degradation into Less toxic compounds |