الفهرس | Only 14 pages are availabe for public view |
Abstract Bone is the basic unit of the human skeletal system. It is continuously remodeled through bone resorption by osteoclasts and bone formation by osteoblasts. An imbalance between bone resorption and formation can result in bone diseases including osteoporosis. There are more than 200 million individuals with osteoporosis worldwide, and the fracture risk of patients with osteoporosis is as high as 40%. About 30 % of patients with long-term (over 6 months) use of GC acquired osteoporosis. Glucocorticoid- induced osteoporosis (GIOP) has become the most common secondary osteoporosis in adults. Bisphosphonate drugs: such as alendronate, are recommended as the firstline therapy for the prevention and treatment of generalized glucocorticoids induced osteoporosis as it suppresses osteoclast activity and slow bone loss. Even though, there is still a controversy concerning the benefits of bisphosphonates versus their long term reported complications. Methyl sulfonyl methane (MSM) is an organosulfur molecule naturally occurring in the human body and many foods. It has antioxidant, antiinflammatory, anti-atherosclerotic, antiapoptotic and antimicrobial activities. The present study was performed to study the effect of Methyl Sulphonyl Methane on the osteoporosis that was induced experimentally by glucocorticoids in adult male albino rat model compared to alendronate sodium. The current study was conducted on Seventy-two healthy adult male albino rats with average weight of 180-200 gm. The rats were classified into six experimental groups: group I: this group included 12 adult male albino rats and was subdivided into 2 subgroups: Subgroup (Ia) (Plain control group): it included six rats and was kept without any treatment all over the experimental periods and they were served as control group for all experimental groups. Subgroup (Ib) (Vehicle-control group): it included six rats. Each rat received 0.2 ml normal saline oral by gavage for 8 weeks. group II (MSM group): this group included 12 rats. Each rat received 0.2 ml normal saline oral by gavage for 4 weeks then received Methyl Sulphonyl Methane (MSM) (400 mg/kg/day dissolved in 1ml normal saline; about 80 mg/rat dissolved in 0.2 ml normal saline) orally by gavage for another 4 weeks. group III (Alendronate group): this group included 12 rats. Each rat received Each rat received 0.2 ml normal saline oral by gavage for 4 weeks then received Alendronate (1 mg/kg/day dissolved in 1 ml normal saline; about 0.2 mg dissolved in 0.2 ml normal saline/ rat) orally by gavage for another 4 weeks group IV (Glucocorticoid Induced Osteoporosis group): this group included 12 adult male albino rats. Each rat received received intramuscular injection of dexamethasone (7 mg/kg, once/week; about 1.4 mg/ rat or about 0.35 ml of dexamethasone ampoule) for 4 weeks to induce osteoporosis then each rat received 0.2 ml normal saline oral by gavage for another four weeks to collect specimens at the end of the experimental period (8 weeks). group V (Glucocorticoids plus MSM treated group): this group included 12 rats. Each rat received intramuscular injection of dexamethasone (7 mg/kg, once/week) for 4 weeks then each rat received Methyl Sulphonyl Methane (400 mg/kg/day; about 80 mg/rat) dissolved in 0.2 ml normal saline for 4 weeks orally by gavage for another 4 weeks. |