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Abstract Biosecurity has emerged solution to a crucial problem for the farming sector as a result of challenges with foreign and exotic diseases as well as growing public concerns regarding food safety. The purpose of this study was to have a look at the biosecurity status of four commercial broiler chicken farms in Sharkia governorate, Egypt. The first farm is in El-Salheya, the second in El-El-khattara, the third in Kafr Saqr and the final in Awlad Saqr. For nine months, from (November 2021 to July 2022), a total of eighty-four samples were collected from each farm (21 each), including water, feedstuffs, litter, cloaca of poultry, wall of poultry houses, hand of worker and foot boot samples (3 each). All collected samples received bacterial analysis in order to identify the presence of E. coli and Salmonella species as biosecurity indicators. Furthermore, the recovered E. coli and Salmonella bacteria were serotyped and their susceptibility to routinely used antibiotics and disinfectants was determined. |