الفهرس 
Introduction and rationaleOnly 14 pages are availabe for public view
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Abstract
One of the main factors contributing to morbidity and mortality across the globe is acute myocardial infarction. To reduce death rates, therefore, efficient and accurate diagnostic biomarkers are needed. This study aimed to evaluate the association of chemokine (C-C motif) ligand18 (CCL18) and expression of miRNA-499-5p in acute myocardial infarction and its component traits among Egyptian populations.
This study was performed on 150 subjects, divided into 75 healthy subjects and 75 patients with AMI. Patients were selected from cardiac intensive care unit at Suez Canal University Hospital, Ismailia, Egypt.
Detailed history includes name, age, marital status, address, occupation, special habits of medical importance e.g., smoking, diabetes mellitus, hypertension and drug in take was documented.
Venous blood samples (5 ml) were drawn. The whole blood sample was divided into two parts:
1. Two millilitres of whole blood were collected into potassium EDTA tubes and used to measure plasma level of CCL 18 using ELISA Kit.
2. The remaining three milliliters were collected into plain tubes and used for serum separation for RNA extraction and measurement of fasting blood glucose (FBG), cardiac enzymes [creatine kinase (CK), creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and cardiac troponin I (CTnI)], and kidney functions (serum creatinine, sodium, and potassium).
Serum miRNA-499-5p expression was determined using qRT-PCR technique and normalization was done against RNU6B as an internal control.