الفهرس | Only 14 pages are availabe for public view |
Abstract A total of 450 table egg samples were collected randomly from different markets in Ismailia Governorate during October 2021. Each farm and baldy eggs are divided in to 60 batches of each (3 eggs), and organic eggs are divided into 30 batches only. All samples were analyzed for detection of oxytetracycline , ciprofloxacin and gentamicin residues using LC – MS/MS method. 1- Oxytetracycline residues in examined egg samples: The level of oxytetracycline in farm eggs ranged from 60.5to 88 ppb with a mean value of69.95 1.78 ppb , but in baldy eggs ranged from 31 to 69.5 with mean a value 43.6 2.65ppb and N.D from any examined organic egg samples. (20 %) of examined farm egg and baldy egg were contaminated with oxytetracycline. According to Codex Alimentarius Commission, all examined samples were below the permissible limit. 2-Ciprofloxacin residues in examined egg samples: The mean level of ciprofloxacin in farm eggs, baldy were 26 0.21 and 87.66 15.66 ppb in the farm and baldy egg samples ,respectively. But in organic egg samples non detected. According to European Commission 20 % in farm egg samples and 60 % of baldy eggs are above the permissible limits, Also ciprofloxacin non detected from any examined organic egg samples. 3- Gentamicin residues in examined egg samples: Gentamicin residues non detected from any examined egg samples (farm, baldy or organic eggs). All examined samples (100%) were accepted. from obtained results, all examined samples for oxytetracycline and gentamicin residues were below the permissible limit. But 10% of examined farm eggs and 60% of examined baldy eggs were contaning with ciprofloxacin residues and unaccepted. -Distribution of oxytetracycline and ciprofloxacine in albumin and yolk after oral administration: Two groups of laying hens (6 of each), first group given oxytetracycline by dose 40mg/kg bw and the second group given 10mg/kg bw ciprofloxacine for 5 days. Egg collected in the 6 day for determination of both drug residues in albumin and yolk. The results showed that oxytetracycline concentration in albumin was 750 ppb but in yolk 500ppb. Also, ciprofloxacine residues were 500ppb and 319 ppb in albumin and yolk , respectively. -Effect of boiling for 15 minutes on both antibiotics residues in albumin and yolk contents: The results showed that the concentration of oxytetracycline and ciprofloxacine in albumin were 93 ppb and 49ppb with reduction percentage equal 87 and 90.5 respectively. But , the concentration in yolk were 50ppb and 40ppb with reduction percentage 90 and 80, respectively. Effect of frying for 5 minutes in the concentration of oxytetracycline and ciprofloxacine (whole egg): -The results showed that after frying of the egg the concentration of oxytetracycline was 71.5 ppb but ciprofloxacine concentration was 45 ppb with reduction equal94.3% and 94.6% , respectively. The economic importance and public health significance of antibiotic residues in table eggs were reviewed . In addition control and prevention of producing safety table eggs and to safeguard the consumers were suggested. |