الفهرس 
AbstractOnly 14 pages are availabe for public view
 |  | from | 86 |  |  |
| | | from | 86 | | |
Abstract
Background: Adipose tissue has a significant expression of the enzyme 11- hydroxysteroid dehydrogenase type 1 (11-HSD1), which functions as a reductase. (to convert inactive cortisone to active cortisol). It has been proposed that the pathogenesis of obesity may be influenced by tissue-specific deregulation of cortisol metabolism. Numerous studies have assessed HSD11β1gene polymorphisms and obesity risk. The evaluation of the relationship between the expression of the HSD11 gene and the risk for obesity and its associated diseases is still lacking in the written literature. Objective: This study was carried out to investigate the relationship of 11β HSD1 gene expression with obesity, diabetes, and hypertension in subcutaneous adipose tissue. This study also correlates 11β-HSD1 gene expression with various parameters in obese patients.
Patients and methods: Our study was conducted on 74 subjects; they were selected from kasr Al Aini Hospital, Surgical Department. They were divided into two groups: 56 obese patients with BMI ≥30 kg/m2 (Obese group) beside 18 age- and sex-matched normal weight healthy subjects (Control group). The expression of 11β-HSD1 was evaluated with real-time PCR.
Results: There was a statistically significant difference between obese and controls in terms of HSD11β1 gene expression level and also there was a significant difference in obese patients with diabetes and hypertension compared with normal weight healthy controls. Furthermore, the correlation and regression analysis showed that the 11β- HSD1 gene expression was correlated with fasting blood glucose and triglycerides levels in obese patients.
Conclusion: The study shows a significant association between subcutaneous adipose tissue HSD11ß1 gene expression and obesity. Moreover, the HSD11ß1 gene expression level is significantly increased the risk of diabetes mellitus and hypertension in obese individuals. Finally, the subcutaneous adipose tissue expression level of 11β-HSD1 gene was correlated to fasting plasma glucose level and also correlated to triglycerides. Keywords: 11βHSD1, gene expression, obese, diabetes mellitus, hypertension.