الفهرس | Only 14 pages are availabe for public view |
Abstract The antibacterial effects of six antibiotics, green synthesized silver nanoparticles (AgNPs), and eighteen plant essential oils were tested against two bacterial strains namely: Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027. Green synthesized silver nanoparticles using Moringa oleifera leaf extract were characterized using UV‒visible spectroscopy, FTIR, X-ray diffraction, and TEM investigation. The results of characterization confirmed that the produced particles were Ag2O and metallic Ag with an average size ranged between 24 to 40 nm. Active antibiotics, essential oils and green synthesized silver nanoparticles were incorporated within bacterial cellulose (BC) and filter paper (FP) discs and their antibacterial activities were evaluated. The results of incorporation showed the efficiency of both bacterial cellulose (BC) and filter paper (FP) discs when incorporated with active antibiotics, essential oils and green synthesized silver nanoparticles against S. aureus and P. aeruginosa Quantitative HPLC found many phytochemicals in cinnamon essential oil, and qualitative GC/MS estimated the prominence of cinnamaldehyde and cinnamic acid as the two primary components in the cinnamon essential oil leaf extract. Furthermore, the Fourier transform infrared spectroscopy (FTIR) verification did not detect any interacting chemical bonds between bacterial cellulose and cinnamon, tea tree, lavender, rosemary essential oils, and green synthesized AgNPs. There were noticeable effects of cinnamon essential oil and green synthesized AgNPs detected on bacterial cell viability, cell integrity, and cell permeability when estimated. Also, transmission and scanning electron microscopy investigation showed major internal and morphological distortions effects of cinnamon essential oil and green synthesized silver nanoparticles on both two bacterial cells tested. |