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Abstract
Total RNA was isolated for deformed wing virus (DWV) and Kakugo virus (KV) from honeybees. Viral detection and identification was confirmed by sequencing of the PCR products using conventional RT - PCR. Homology search of DWV PCR amplified fragment revealed similarities of 99% with DWV isolate Warwick - 2009 poly - protein gene (accession number: GU109335.1) and 98% with DWV isolate Chilensis A complete genome (accession number: JQ413340.1). Also, homology search of KV PCR amplified fragment revealed similarities of 97% with KV isolate - Ox genomic sequence (accession number: KC786224.1), 90.9% with DWV - Ox genomic sequence (accession number: KC786223.1) The described real - time RT - PCR SG RT - PCR proved to be a fast, accurate and useful technique to detect and quantify low viral loads in honeybees and varroa mites