الفهرس | Only 14 pages are availabe for public view |
Abstract Abstract The purpose of this study was to create a new faba bean genetic database simple sequence repetition (SSR) primers and categorise them based on the genes and biological processes that they target. To find probable SSRs, researchers analysed previously published 75,605 and 148,196 faba sequences in the genome and transcriptome, respectively. The total number of SSRs found in each dataset was 25,502 and 12,319, respectively. Trinucleotides have the most repeat counts, followed by dinucleotides, according to the distribution of different repeat classes. Only 238 (21.8%) of the 1091 PCR primers were successful designed from the recovered genic SSR sequences target genomic sequences, while the remaining 853 PCR primers target transcriptome sequences. Our SSR primers addressed roughly 897 genes, according to the annotation of gene-targeted SSRs. There are around 1890 gene ontology (GO) identification codes available. The GO keywords were dispersed across many molecular cell characteristics. There were 554 technical words, 196 domains, and 160 molecular feature phrases with the most redundancy. The molecular function, cellular component, and biological process were among the GO codes that pertained to the general level of GO (544, 670, and 676 GOs, respectively). To test 12 Egyptian faba bean genotypes, 27 SSR PCR primers were created. Approximately 11 SSRs produced one to two PCR bands, whereas some only produced one sharp band with a varied band size. A total of 13 polymorphic primers were utilized in this study. The information content of the polymorphism was 0.3, indicating that it was fairly informative. Five ISSR and six SCoT primers were used to investigate the similarity and relationship among the twelve faba bean genotypes, gave positive results. Using ISSR generated a total of (71 bands) with an average of 1 |