الفهرس 
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Abstract
Aim of the work: To detect the prevalence of Klebsiella pneumoniae sequence type 258 in the clinical isolates collected from patients at Fayoum University Hospitals as recommended by the infection control unit and to detect the antibiotic resistance patterns, the phenotypic and genotypic characterizations of these isolates.Methods: We collected the bacteriological samples then we did bacteriological isolation and identification of K. pneumonaie isolates. Then we screened the K. pneumonaie isolates for detection of carbapenem resistant K. pneumonaie Strains. Then the (CRKP) isolates were subjected to antibiotic susceptibility testing and genotypic and phenotypic characterization. Finally, we detected the prevalence of K. pneumoniae ST 258 by polymerase chain reaction technique through detection of pilv-1 and prp genes.Results: Out of 200 confirmed K. pneumonaie samples, 49 samples were CRKP isolates. According to the MIC results of the CRKP isolates, all the isolates were considered MDR and (53.1%) of the isolates were considered XDR. We detected the prevalence of genes encoding important b-lactamases (blaSHV, blaTEM, blaOXA-1, blaCTX-M) among the CRKP isolates. We also detected the prevalence of acquired carbapenemase genes (blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48) among the CRKP isolates. In our Study we detected (16.3%) of the CRKP isolates harboring both pilv-I and prp genes. These 8 isolates were blaKPC positive and so we considered them as KPC- producing K. pneumoniae ST 258.Conclusions: Antibiotic resistance is a rising threat in Egypt.
Key words: K. pneumonaie ST258, CRKP, blaKPC, pilv-1 gene, prp gene.