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العنوان
Occurrence and risk factors associated with pathogenic E. coli in diarrheic calves /
الناشر
Safaa Abdelmoneim Mohammed Mostafa ,
المؤلف
Safaa Abdelmoneim Mohammed Mostafa
هيئة الاعداد
باحث / Safaa Abdelmoneim Mohammed Mostafa
مشرف / Jakeen Kamal Abdelhalem Eljakee
مشرف / Sherif Abdelmoneim Omar
مناقش / Mona Ibrahim ElEnbaawy
تاريخ النشر
2019
عدد الصفحات
84 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
3/8/2019
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

from 108

from 108

Abstract

Bacteriological examination of fecal swabs collected from healthy (H) and diarrheic (D) calves, revealed isolation of 26 E. coli isolates from 56 calves with an incidence (46.4%). The serological tests for representative number of samples from every farm identified O1, O26, O44, O55, O115, O119, O125, O146, O151 and 2 samples were untyped. The isolates were sensitive to norofloxacin and gentamicin with 76.9% and resistant to Ampicillin and Cefotaxim with 100%.The PCR for 4 gene ompA, stx1, stx2 and eaeA, detected ompA gene in all 26(100%) fecal samples, multiplies a region of 919bp, stx1 gene multiplies a region of 614pb in 1(3.8%) isolate, eaeA gene was in 5 (19.2%) isolates, and no evidence for presence of stx2 in all 26 E. coli isolates. Four colostrum samples were positive for E. coli. The isolates were serogroups O26, O55 and O119. All isolates were negative for eaeA, stx1 and stx2 except strain number 4 (O55) was positive for stx1 only