الفهرس 
Title : Detection of IDH1 and IDH2 mutations in denovo acute myeloid leukemia patients at National Cancer Institute, Cairo University
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Abstract
Introduction: AML is characterized by the uncontrolled clonal proliferation of poorly differentiated cells of myeloblasts. Isocitrate dehydrogenases (IDH1&2) are enzymes involved in multiple metabolic and epigenetic cellular processes. Mutations in IDH1 or IDH2 induce production of an oncometabolite, 2-hydroxyglutarate (R-2-HG) which leads to DNA hypermethylation, and abnormal differentiation. IDH mutations diversely affect prognosis of patients with AML based on the presence of other co-occurring genomic abnormalities. Aim of the work : The aim of this study was to assess the frequency of IDH1& IDH2 genes mutations in AML patients and evaluate their role in disease prognosis. Patients and Methods: We analyzed IDH mutations in 70 denovo AML patients (exon 4 for IDH1& exon 4 for IDH2) using High Resolution Melting (HRM) curve analysis