الفهرس 
Propagation of the rare ornamental climber porana panculata by tissue culture
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Abstract
This work was carried out at ornamental horticulture department, Faculty of agriculture, Cairo university and the experimental work was carried out at the tissue culture laboratory, horticulture research institute, agricultural research center, Giza, Egypt during the period from 2015 to 2017, to investigate some factors affecting the propagation of the rare ornamental climber Porana paniculata by tissue culture. Results could be briefed in the following: During the establishment stage, explants exposed to Clorox at 20% for 15 min obtained the highest position in survival %. Using 2,4-D at 1.0 ppm + BAP at 2.5 ppm occupied the 1st position in callusing %, callus fresh weight and callus diameter. Applying 2-iP at 3 ppm + 1/2 MS medium produced the highest values of callus survival %, morphogenic callus %, culture efficiency, shoot number, shoot length and leaf number. In the multiplication stage, using BAP at 0.60 ppm + 1/2 MS medium resulted in the highest records of lateral buds survival %, regeneration capacity, culture efficiency, shoot number, shoot length and leaf number. BAP at 1 ppm + NAA at 0.1 ppm recorded the 1st position concerning shoot survival %, shoot number, shoot length and leaf number. Concerning the rooting stage, the 1st position for survival %, root number and shoot number was obtained when combinations of BAP plus IBA at either 1 or 3 ppm were used