الفهرس | Only 14 pages are availabe for public view |
Abstract This work was carried out at the Tissue Culture Laboratory, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Giza, Egypt during the years of 2018 and 2021 on the commercially important fern Davallia fejeensis Hook and Nephrolepis exaltata Schott cv. Bostoniensis to establish a protocol of micropropagating for these plants. Explants sterilized with 0.1% MC for 15 min was the sutabit concentration and time for contamination-free and survival percentage in both ferns. During the multiplication of D. fejeensis, the greatest leaf number, shootlet number and heaviest weight were observed in explants cultured on MS medium with kin at 1.0 ppm. The highest total chlorophyll was recorded with 0.5 ppm kin + 0.5 ppm NAA while carotenoid raised in control medium, 0.5 ppm BAP or kin. For rooting, MS containing 4.0 ppm NAA produced the highest number of root and rhizome/ pantlet and longest rhizome. For acclimatization of the fern plantlets cultured in peat moss +sand +perlite (1: 1: 1) produced the longest plantlet, greatest leaf number, fresh weight, root number, rhizome number and longest root, rhizome. For the multiplication of N. exaltata , the highest shootlet number and leaf number were recorded in MS supplemented with 1.0 mg/l BAP and 1 g/l AC. For rooting, full MS with 0.5 mg/l NAA and 1 g/l AC produced the highest rooting percentage, root number, and longest root. For acclimatization, culturing plantlets in peat moss recorded the longest plantlet, greatest leaf number and longest root. |