الفهرس 
INTRODUCTION AND AIM OF THE STUDYOnly 14 pages are availabe for public view
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Abstract
In the present study effort have been made to reports on the evaluation of chemical composition, antioxidant and antimicrobial activities of three Egyptian medicinal plant essential oil namely, Foeniculum vulgare (Fennel), Pimpinella anisum (Anise oil) and Mentha spicata (Spearmint oil). Medicinal plants samples used in this study located in the Egyptian market, which is a leaves of spearmint plant and the dry fruits of anise and fennel plants. The volatile oils were extracted by water distillation and chemical composition analysis by GC\MS. As it has been stood.
The principal components of essential oils were trans-Anethole (83.43 %) for F. vulgare, carvone (36.04%) for M. spicata and trans-Anethole (91.80%) for P. anisum. The antimicrobial activity to those derived oils were assessed against therefore bacteria; including three recombinant strains of Escherichia coli: E.coli JM109, E.coli JM109 (DE3) and E.coli (DE3) BL21 PLysS, two strains of Bacillus; Bacillus licheniformis SHG10 and Bacillus cereus, four strains of pathogenic bacteria; Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Salmonella typhimurium and E.coli enterotoxigenic. Using the diffusion method Disc and calculating the diameter of inhibition zones. The antibacterial activity was measured in terms of zone of growth inihibition in mm. A statistical analysis of the results obtained has been constructed.
Three essential oils understudy exhibited considerable inhibitory effects against all organism understudy. The essential oil show the strong growth inhibiting activity was found in spearmint oil.
The results of the antibacterial activity of essential oils on bacteria were compared with them and the known type of Industrial antibiotics; Cephradine (30 µg/disc) and Tobramycin (10 µg/disc), and it showed various levels of antimicrobial activities.
On the other hand, Standards of Synthetics commercial principal Components of Fennel, Anise, and Spearmint, were test its antimicrobial activity against selected indicator microorganisms using the same essential oil, to comparison between the effect of extracted crude oils and their main constituent against the indicator microorganism was also carried out and revealed variable results.
The Minimum Inhibitory Concentration of Anise, Spearmint and Fennel oils was measured by prepared serial dilutions using DMSO and test their antimicrobial activity against the indicator strains using the agar well-cut diffusion technique.
In the other side, the antioxidant activity of The three essential oils understudy were studied, the essential oil show the strong antioxidant activity was found in anise oil which presented the best antioxidant profile, given its highest percentage % of inhibition of DPPH radical (64.28%). The microbial effect to those derived oils using the diffusion method Disc and calculating the diameter of inhibition zones. A statistical analysis of the results obtained has been constructed.