الفهرس 
INRTUDUCTION & AIM OF THE WORKOnly 14 pages are availabe for public view
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Abstract
Abstract
This study was designed to evaluate and compare the efficacy of the mesenchymal stem cell -derived microvesicles (MSCs- MVs), injectable platelets rich fibrin (I-PRF) and advanced platelets rich fibrin (A-PRF) for enhancement of the healing of transected Achilles tendon (AT). As well as, to evaluate the efficacy of ultrasound and MRI as non-invasive diagnostic tools with the histopathological examination for monitoring the healing process of AT. Twenty-four adult clinically normal mongrel dogs weighing 18-20 kg were used in this study. All animals were subjected to transection and surgical repair of Achilles tendon using modified kessler’s tenorrhaphy. Dogs were divided into four equal groups including Control group, A-PRF group, I-PRF group and MVs group. During the period of observation, dogs was observed clinically and ultrasonographically (at the 3rd, 6th, 8th ,10th and 12th week). By the end of the 6th and 12th weeks, the operated tendon was harvested after animal euthanasia and subjected to morpho-histological evaluation. At the end of the study and before euthanasia, the remaining animals were subjected to MRI examination and scoring systems have been used for monitoring the healing process and complete evaluation of the tested biomaterials. Results revealed that, MVs and I-PRF treated groups had the upper hands on the healing process of the tendon throughout the study period while A-PRF had better healing enhancement than the control group but still lower than the MVs and I-PRF groups. This study is considered as prelude for future use of MVs and I-PRF products in various clinical applications of tendon affection in animals and humans.
Key words: Achilles Tendon, A-PRF, I-PRF, Mesenchymal stem cells, Microvesicles