الفهرس 
Recent updates of Lymphoid NeoplasmsOnly 14 pages are availabe for public view
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Abstract
Although, Fine-needle aspiration cytology remains the first diagnostic aid in pathologies of lymphadenopathy some technical and diagnostic drawbacks also exist. Therefore, additional investigations are usually required specifically flow cytometry that remains the best way to identify clonality and markers’ expression. Aim of the work: this study aimed to assess the value of cytomorphology and multiparametric flow cytometry in the diagnosis and classification of reactive lymphoid hyperplasia and malignant lymphoma using lymph node samples. Methods: In this cross – sectional study 40 adult patients presented with lymphadenopathy were included. Fine needle aspiration (FNA) cytology, immunohistochemistry and flow cytometric (FCM) analyses were performed. Results: A highly statistical kappa agreement was obtained between results of FCM and IHC as regard the diagnosis of benign and malignant disorders in addition to discrimination between Hodgkin and non-Hodgkin lymphomas and their subtypes (k=0.719, k=0.759 and k=0.809 respectively). Evaluation of FCM performance showed diagnostic sensitivity and specificity of FCM was found to be 73.1% and 100% respectively compared to 80% and 100% when combined to cytomorphology in diagnosis of all studied patients. Furthermore, combination of cytomorphology and FCM showed a sensitivity of and specificity 100% in differentiating between B and T NHL cases. Conclusion: FCM coupled to cytomorphology has high sensitivity and specificity allowing reliable diagnosis of benign and malignant causes of lymphadenopathy together accurate identification of different types of lymphomas.