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Abstract Aflatoxin M1 is one of mycotoxin derivatives, which is secreted in milk of dairy cattle fed on feed contaminated with Aflatoxin-B1 (AFB1). Following dairy cattle ingestion of AFB1 -contaminated feed, AFB1 is rapidly adsorbed and transported to the liver where it is partially metabolized into the hydroxy-derivate M1 (AFM1) that is secreted in milk.AFM1 is considered as toxic as AFB1 and has been included in group 2 toxins that are potentially carcinogenic for humans. The current study was designed to prepare a vaccine against AFB1 and to evaluate its efficacy in reducing or preventing secretion of AFM1 in milk.Aflatoxin-B1 was prepared, purified and transformed into oxime.Then it was fixed on bovine serum albumin as carrier molecules to become immunogenic.The AFB1-BSA conjugate was adjuvanted with Gold Nano particles (AFB1-BSA-GNPs)and Montanide ISA 206.The prepared AFB1-BSA-GNPs vaccine was used for immunization of rabbits and dairy cattle. In rabbits the vaccine was given S/C in a dose of 100og/ml. In cattle the vaccine was given I/M in a dose of 500ug/2ml. The vaccinated animals were boosted with a second dose 3 weeks post primary immunization.The vaccinated and control rabbit groups were bled 2 weeks after primary vaccination and at 2, 4 and 6 weeks post booster vaccination |