الفهرس 
Title : Investigation into the use of SeM protein as immunogenic material against equine strangles disease
ContentsOnly 14 pages are availabe for public view
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Abstract
SeM protein extracts were prepared from locally isolatedS.equi subspecies equi. Modification by further centrifugation of both the supernatant and sediment of the SeM acid extract was made. The resulting supernatants of both centrifugations were analyzed electrophoretically using SDS-PAGE, including SeM acid extract sediment (SeM1), SeM acid extract supernatant (SeM2), supernatant from centrifuged SeM1 (SeM3), and supernatant from centrifuged SeM2 (SeM4). Electrophoresis of SeM1 revealed the appearance of a band at MW 70.9 kDa, While SeM2 revealed the presence of 7 bands at MW of 105, 87.8, 70.9, 61.1, 44, 37.9 and 18.4 kDa. Five bands were detected in SeM3 at MW 70.9, 58.9, 37.2, 29.8 and 18.3 kDa,and SeM 4 showed four bands at MW of 72.0, 58.6, 29.8 and 18.0 kDa.Antibody titer was detected in the sera of rabbits immunized by SeM3 and SeM4 using indirect ELIZA