الفهرس | Only 14 pages are availabe for public view |
Abstract Tomato (Lycopersicon esculentum Miller, family Solanaceae) is one of the most important vegetables worldwide because of high consumption of fruits, year round availability and large content of health related components. It has been extensively studied by several workers on the plant growing abroad; however, few studies have been focused on the leaf, stem and root.These organs are discarded after harvesting, considered as waste products and worthless for industrial processes. Our study was carried out on tomato plant waste (leaf, stem and root) cultivated in Egypt for investigation of biologically active metabolites in an approach to find any medicinal benefits from such waste product. DNA fingerprinting and botanical studies help for identification and authentication of the plant. Preliminary phytochemical screening of the different organs was carried out. The lipoidal content of the leaf and stem were subjected to GC/MS analysis. Eight compounds were isolated and identified in the leaf as: apo-Ý-carotenol, Ý-sitosterol, protocatechuic acid, stigmasterol 3-О-Ý-D-glucoside, tomatidine, Ü-tomatine, quercetin and rutin.Colorimetric estimation of total flavonoids and phenolics content showed that the leaf has the highest percentage followed by the stem then the root. These results were supported by HPLC analysis of flavonoids and other phenolics in the leaf and stem. HPLC determination of Ü-tomatine in the different organs showed that the ethanolic and aqueous extracts of the stem has the highest concentration followed by the ethanolic extract ofthe root then that of the leaf |