الفهرس 
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Abstract
This study had designed to estimate the antioxidant and
hepatoprotective effects of Moringa oleifera and pumpkin seed
oil against hepatic toxicity induced by CCL4 in male albino rats.
Sixty male albino rats with an initial weight of 110 – 130 g.
Before starting the experiment, they were acclimatized for
10 days in animal house. They were randomly divided in
plastic cages into 6 groups. 10 rats for each group in each
cage. (Group1) as (normal control group) rats received
balanced diet only. (Group2) as (Moringa treated group)
rats received 0.2 ml of Moringa oleifera seed oil/rat by oral
gavage daily for 28 days. (Group3) as (pumpkin treated
group) rats received pumpkin seed oil at the dose of 300 mg
/kg body weight by oral gavage daily for 28 days. (Group4)
as (CCL4-intoxicated group) rats received CCL4 i/p at a
dose of 2.5 ml/kg b. wt. dissolved in corn oil (30%) on day
13 and 14 of the experiment. (Group5) (Moringa + CCL4
group) rats received 0.2 ml of Moringa oleifera seed oil/rat
by oral gavage daily for 28 days with i/p injection of CCL4
on day 13 and 14 of the experiment. (Group6) as (pumpkin
+ CCL4 group) rats received pumpkin seed oil at the dose of
300 mg/kg b. wt. by oral gavage daily for 28 days with i/p
English
Abstract
injection of CCL4 on day 13 and 14 of the experiment.
The antioxidant and hepatoprotective effects of Moringa
oleifera and pumpkin seed oil against hepatic toxicity
induced by CCL4 were explored by assessing hematological
parameters, liver enzymes levels in serum: alanine
aminotransferase (ALT), aspartate aminotransferase (AST),
and alkaline phosphatase (ALP); total protein, albumin,
globulin, A/G ratio, total cholesterol, TG, HDL, LDL,
VLDL, and antioxidant enzymes: glutathione reductase
(GSH), catalase (CAT), and malondialdehyde (MDA), and
histopathological examination of liver tissue.
Results: The results revealed that oral administration of
Moringa oleifera seed oil and pumpkin seed oil in CCL4
intoxicated rat for 28 days effectively ameliorated
hematological parameters, markedly decreased the
increased levels of (ALT, AST, ALP, total, direct, and
indirect bilirubin and elevated serum levels of total protein,
albumin, and globulin and also showed a marked
improvement in lipid profile compared with CCL4
intoxicated group. Glutathione reductase, and catalase were
markedly increased while malondialdhyde was markedly
decreased compared with CCL4 intoxicated group. Liver
sections of rats pretreated with Moringa and pumpkin
histopathological examination showed favorable changes in
degenerative changes created by CCL4.
Conclusion: These results advise that oral administration of
Moringa oleifera and pumpkin seed oil have potent
antioxidant and hepatoprotective effect against CCL4
intoxicated rats. This research recommends that oral
administration of moringa and pumpkin seed oil may be
favorable for patients with oxidative stress and liver
disorder.