الفهرس 
Title: Advanced studies on infected calves with diarrheagenic Escherichia coli
AbstractOnly 14 pages are availabe for public view
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Abstract
Escherichiacoli is implicated as a major cause of diarrhea in young calves with significant economic losses worldwide. The emergence of antimicrobial resistance within E. coliis considered a major challenge to veterinary and public health. The phenotypic and genotypic characteristics of antibiotic multi-resistance within different E. colipathotypes obtained from diarrheic calves have been performed. Furthermore, the role of M.domestica and milk has been identified in the transmission of pathogenic multi-resistant strains. A total of 213 E. coli strains were isolated from 157 diarrheic calves with molecular detection of ETEC, EPEC, STEC and AE-STEC at 13.6%, 14.1%, 15.5% and 13.1%, respectively. Hybrid pathotypes of ETEC/EPEC, ETEC/STEC and ETEC/ AE-STEC were identified at 3.3%, 5.2% and 3.8%, respectively. Molecular detection of AE-STEC O157 strains was performed on 137 rectal swabs from 157 diarrheic calves and was presented at 8.02%. Virulence genes profile of AE-STEC O157revealed the presence of eae, stx1, stx2 and ehylA in percentages of 93.3%, 73.3%, 20% and 13.3%, respectively with the predominance of eae+stx1 combination at 66.7%.The association of M. domestica and milk with the transmission of pathogenic multi-resistant strains was determined on 110, 80 and 26 E. coli strains isolated from 70 rectal swabs from 157 diarrheic calves, 60 milk samples and 20 M. domestica, respectively from dairy farms located on the Giza and Cairo-Alex desert road. Molecular pathotyping of these strains revealed the detection of pathogenic E. coli within diarrheic calves, M. domestica and milk at 81%, 76.9% and 33.7%, respectively