الفهرس | Only 14 pages are availabe for public view |
Abstract In this study 100 samples of raw buffalo milk, raw cow milk, kariesh cheese and raw cream (25 of each) were randomly collected from different markets, groceries, villages and dairy farms at Dakahlia Governorate, Egypt. The samples were examined for detection of total bacterial count, most probable number of coli forms, total Enterobacteriaceae count and isolation of E. coli, Salmonella and Staphylococcus aureus.The highest frequency distribution of total bacterial count lied within the range of (106-107) in the examined samples of raw buffalo milk (44%), raw cow milk (52%), raw cream (48%).The highest frequency distribution oftotal coliform countlied within the range of (105-106) in the examined samples of raw buffalo milk (44%), raw cow milk (36%), raw cream (10%) and kariesh cheese (10%) based on their total coliform count MPN /mL or g. The E. coli was detected in 84%, 76%, 92% and 68% in examined buffalo milk, cow milk, kariesh cheese and raw cream, respectively. Among these isolates obtained from the raw milk and milk product samples in this study, we found that genes conferring resistance to tetracycline tetA was detected in 8/10 strains. The S. aureus detected in 44%, 36%, 60% and 48% in examined buffalo milk, cow milk, kariesh cheese and raw cream, respectively. In this study, the mecA gene was successfully amplified in 5 out of 10 tested isolates (50 %) at 310 bp.The overall occurrence of biochemically identified Salmonella from raw milk and dairy products on XLD was 3% (3/100), distributed as 4% (1/25), 8% (2/25) among milk, raw cow milk and kariesh cheese, respectively. Based on these obtained result it seems necessary that concerned authorities should impose regulations and bacteriological standards to govern raw milk and its products. |