الفهرس | Only 14 pages are availabe for public view |
Abstract Osteoarthritis (OA) is the most common degenerative disorder of synovial joints marked by articular cartilage erosion. The majority of current therapies targeted only OA symptoms, and still there is a need for treatments that decrease pain and improve the cartilage function. Alginates (ALG) are a family of naturally abundant anionic polysaccharides extracted from the cell walls of the brown algae, they are one of the increasingly studied ones in the biomedical and pharmaceutical researches and its monograph is included into both the European Pharmacopeia and the United States Pharmacopeia This study directed to show the alginate ameliorative effects on MIA-induced osteoarthritis in rats, Our study was carried on 77 rats divided into three groups as follows group 1: 28 male rats apparently healthy representing the control group, group 2: 28 male rats were subjected to a single intra- articular dose of MIA , group 3 : 21 male rats treated with alginate Histopathological results showed that Intra-articular injection of MIA by 2 weeks, induced joints arthritis which had a mild focal fissuring in the upper third, disorganization of chondrocytes and focal chondroid hyperplasia and Neovascularization of the matrix, with an increase in aggrecanase, β-glucuronidase, and hyaluronic acid and a decrease in the level of glutathione when compared with that of the control Reduced glutathione levels were found to be significantly decreased in rats with osteoarthritis than in control due oxidative stress involved in pathogenesis of OA which results due to elevated free radical production. Histopathological examination from our study evident that the alginates produce antiinflammatory effect which showed by the high decreases in the enzymatic activities level of β-glucuronidase and aggrecanase and hyaluronic acid Alginate treatment results in reduction of the enzymes aggrecanase, β-glucuronidase which indicate decrease in cartilage degradation. 6.2. Recommendations (1) Our present study recommended that the Brown algae could produce an ameliorative effect on OA. (2) Further studies should be done in mammals to provide more insights into the underlying mechanisms and to determine the highly effective components in alginate that could have maximum therapeutic efficacy with minimum side effects. (3) The inflammatory markers can be used to predict disease development and treatment success such as ( β glucouronidase ) . (4) Hyaluronic acid and aggrecanasecan be excellent biomarkers of osteoarthritis progression. |