الفهرس 
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Abstract
: To investigate and compare the anticancer effect of magnetic selenium nanocomposite alone or after adding titanium or sodium borate on tongue carcinoma stem cells.
Methodology: Cancer stem cells were isolated from tongue carcinoma cell line using CD-44 pluriBead® technology. The cultured cancer stem cells were classified into four groups. group I
received no treatment to represent a control group. Cells of group II received magnetic selenium
nanocomposite. group III cells received titanium- magnetic selenium nanocomposite. While sodium borate- magnetic selenium nanocomposite was administered to group IV cells. All
intervention groups were left to grow with the applied nanocomposite for 24 and 48 hours with application of an external magnet. MTT cell proliferation assay was performed for all groups at 24
and 48 hours, and the results were analyzed statistically. Ultrastructural changes in all study groups were evaluated by transmission electron microscope.
Results: MTT assay results showed that the highest proliferation occurred in group I (control group)
at 48 hours compared to all other groups. At 24 hours, only group II (magnetic selenium group)
showed significant reduction in cell proliferation in comparison to the control group. All intervention
groups showed significant reduction in cell proliferation at 48 hours in comparison to the same groups
at 24 hours and to the control group. group II and group III at 48 hours showed nearly similar results
with statistical insignificance while they had statistically significant difference with other groups.
Ultrastructural examination revealed that apoptotic changes took place in the cancer stem cells of all
vii intervention groups in comparison to the control group. The most aggressive changes appeared in group II followed by group III at 48 hours of treatment.
Conclusion: The three magnetic selenium nanocomposites demonstrated an anticancer effect against tongue carcinoma stem cells in vitro. Their anticancer effect became more efficient with increasing the exposure time to 48 hours. Magnetic selenium nanocomposite alone appeared to be the most promising nanocomposite.