الفهرس 
ACKNOWLEDGEMENT
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Abstract
In the present study, a total of 500 random eggs, representing 100 samples of farms and baladi eggs samples (50 samples of each) were
collected from poultry farms, markets, supermarkets, and groceries
located in Assiut governorate, Egypt. The samples were microbiologically examined, and isolates were identified by biochemical and (PCR) for the presence of coliforms, fecal coliforms, E.coli and Salmonella.
1- Coliform count:
Most probable number (MPN) technique showed that 62, 56, 54 and 32% of the examined samples of farm hens’ egg shell, egg content, baladi hens’ egg shell and egg content were contaminated with coliforms,
respectively.
2- Fecal coliform count :
Most probable number (MPN) technique revealed that 40, 26, 36 and 22% of the examined samples of farm hens’ egg shell, content, baladi hens’ egg shell and content were contaminated with fecal coliforms, respectively.
3- E.coli count :
Most probable number (MPN) technique and biochemical
identification revealed 16 isolates of E. coli and these results were complementary with the molecular identification. E.coli incidence was 6, 8, 12 and 6% for the farm hens’ egg shell, content, baladi hens’
egg shell and content, respectively.
4- Incidence of Salmonella:
In the current study, Salmonella couldn’t be isolated from both farm and baladi hens’ eggs.
The obtained results of the present study proved that most
samples of the farm hens’ eggs and baladi hens’ eggs were highly
contaminated with large counts of coliforms and fecal coliforms while E.coli existed in low incidence. The optained data indicated that baladi hens’ egg shells and contents more contaminated with E.coli than farm hens’ egg shells and contents samples. While, we couldn’t isolate Salmonella from all examined egg samples. Concerning the economic and public health importance of these pathogens that affect the human health through consumption of eggs were discussed. Likewise, the suggestive measures for improving the quality of produced eggs