الفهرس | Only 14 pages are availabe for public view |
Abstract Objective: This study was conducted to evaluate the impact of enamel matrix derivatives (EMD) on dental pulp stem cells. Methods: The effect of enamel matrix on DPSCs was unclearly known, thus this study had been performed. This study was designed into five groups, as group A (control group), groups (B, C, and D) DPSCs were seeded with different concentrations of EMD (1 µg/ml, 10 µg/ml, and 100 µg/ml) respectively. The cells were examined for proliferation and viability by MTT assay at various durations (days 1, 3, 5, and 7). The 5th group was group E (control positive) DPSCs were seeded with osteogenic media for Alizarin Red S staining assay and semi- quantitative real-time PCR evaluation. Semi-quantitative real-time PCR was performed for evaluation of specific genes (dentin sialophosphoprotein [DSPP], alkaline phosphatase [ALPL], and transcription factor [SP7]) and glyceraldehyde 3-phosphate dehydrogenase [GAPDH] was used as a housekeeping gene. Results: Cell viability assay showed up that concentration 100 µg/ml of EMD was the best concentration in cell viability for days 1,3 and 5, but concentration 10 µg/ml was better at day 7. Alizarin Red S staining revealed that the highest concentration was the most effective in the accumulation of calcium-rich nodules. Real-time PCR evaluation show that the highest expression of odontogenic genes (DSPP) was associated with concentration 100 µg/ml of EMD. While the highest expression of osteoblastic genes (ALPL, and SP7) was associated with concentrations (1µg/ml and10 µg/ml) of EMD. Conclusion: Enamel matrix derivative (Emdogain) with highest concentration (100 µg/ml) was considered as the best one in cell viability, calcium deposition, and increased expression of odontogenic gene involved in the current study. |