الفهرس 
Introduction and RationaleOnly 14 pages are availabe for public view
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Abstract
Present study was conducted to characterize the clinical A.baumannii isolate with special reference to the detection of antimicrobial sensitivity and resistance, biofilm formation and molecular detection of different genes that was reported from previous studies to be associated with virulence of A.baumannii.
The study included 43 A.baumannii isolates obtained from outpatients and hospitalized patients admitted in the Urology, Internal medicine, surgical wards, Intensive Care Unit (ICU) and Neonatal ICU (NICU) and attending outpatients’ clinics in SCUHs. Majority (66%) of infections by A.baumannii strains were Healthcare-associated. The majority (72%) of A.baumannii isolates were obtained from the ICU, followed by internal medicine wards (14%). The majority (72 %) of isolates were obtained from ETA and sputum specimens.
Results of antibiotic sensitivity testing of the isolated A.baumannii strains in were obtained by determining the Minimum inhibitory concentration (MIC) values using vitekA1C system. And the results revealed that the highest sensitivity was to Colistin, Tigecycline and Trimethoprim-Sulfamethoxazole with sensitivity rates 90.6%, 73% and 62.7% respectively and the highest resistance rate was towards amikacin (86%), and the majority of isolates were MDR (88.4%).
Biofilm production was detected in 90.6% of the examined isolates using the phenotypic method (tissue culture plate method), 81.4% of the study isolates were MDR and biofilm producers, with statistically significant relation between biofilm production and the MDR phenotype. And 81.4% of the study isolates were biofilm producers and patients were