الفهرس 
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Abstract
Herbs are natural alternatives to antibiotic growth promoters (AGPs) in animal nutrition due to their antimicrobial properties. Many herbs and their bio-active constituents possess a broad antimicrobial activity, and appetite and digestion stimulating effects. Herbal growth promoter (essential oil) had significant improvement of body weight, weight gain, and feed conversion.Thyme can be used traditionally for several medicinal purposes: respiratory disease, antimicrobial and antinociceptive. Supplementation with thyme oil improved the growth performance and antioxidant enzyme activities.Boldenone (BOL) is an androgenic steroid that improves the growth and food conversion in food-producing animals. In most countries worldwide, this anabolic steroid is forbidden for human uses and meat production as it was developed for veterinary use. Recently, BOL is used by bodybuilders in both off season and pre-contest, where it is well known for increasing vascularity while preparing for a bodybuilding contest.The aim of the present experimental study was to investigate the biochemical changes in rabbit blood after sample with Thyme Ginger BOL as growth promoter in white male New-Zeland rabbits.The Animal of the Experimental Study This study was carried out 24 white New-Zealand male rabbits were selected at the age from 6-8 weeks old with an average body weight of 0.600 - 0.750 kg were used in the experimental investigation of this study.Rabbits were housed in individual metal cages. Fresh and clean drinking water was supplied ad-libitum. The animal room was well ventilated with a 12 hours light/dark cycle throughout the experimental period.The rabbits were fed on a basal diet throughout the course of the experiment in the form of concentrated diet composed of 40% Alfalfa meal, 20.25% Ground barley, 20% Wheat mill run, 14% Soybean meal, 3% Molasses, 1.5% Fat, 0.75 Dicalcium phosphate, 0.5% Salt (trace, mineral).Design of the experimental study.Twenty four white New-Zealand male rabbits were selected at the age from 6-8 weeks old with an average body weight of 0.600 - 0.750 kg were used in the experimental investigation of this study. The rabbits were randomly divided into four equal groups, each one consisting of six animals placed in individual cages and classified as follows:group I (control) rabbits received normal commercial basal diet contained. group II (Thyme): rabbits fed normal basal diet contained 5 % thyme (5gm/100gm basal diet).<group III (Ginger): rabbits fed normal basal diet contained 5 % ginger powder (5gm/ 100gm basal diet).group IV (Boldenone): rabbits received boldenone injection as a growth promoter 5% oily solution (Equigan®; Lab Tornel, Co., Mexico) (5ml/Kg. b.wt, injection) two times, the first dosage at the onset of experiment and the second dose after 15 days. Body weights were recorded three times at the beginning of experiment, then after 15 day and at the end of the experiment (30 day).Sampling:Blood sample were taken after 15 day and at the end of the experimental period, after an overnight fasting by vein puncture of the marginal ear vein, centrifuged at 3000 g for 10 min using bench top centrifuge. A serum was separated and collected using dry Pasteur pipette. Labeled and stored in the refrigerator at -20°C for analyses. For measurmet of the following :Biochemical Measurements Serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), Total protein, albumin, , creatinine, lipid profile include Total Cholesterol and triglyceride (TC and TG) oxidative stress marker such as reduced glutathione (GSH) concentration, Pro-inflammatory cytokine (IL-6), testosterone level and growth hormone (GH)The obtained results summarized as the following:1. Live Body weight After one month treatments, the obtained results showed that, there was no statistical significance difference between the rabbits groups in initial body weight in the 1st sample (p = 0.51); but there were statistical significance differences between them in 2nd sample (p< 0.001). The difference was between Ginger group and other groups; but no difference was found between Thyme group and control group. Additionally, there was a significant difference in body weight between BOL group and other groups. There was statistical significantly increase in body weight in 2nd sample between all rabbits groups compared to 1st sample.2. Serum AST and ALP of the different rabbits groups at 1st and 2nd sample follow-up The results of our study revealed that, there were statistical significance differences between the rabbits groups in AST and ALP in both 1st and 2nd sample. According to the investigation of ALP at the 1st and 2nd sample; statistically, no difference was found between thyme and ginger groups. On the other hand, there were significance differences between BOL group and all other groups and between control group and all other groups at the 1st when compared with the 2nd sample; but no significant difference was found between control, ginger or thyme groups. Additionally, there was statistical significantly increase in AST in 2nd sample in BOL group compared to 1st sample. Also there was a significantly increase in ALP in 2nd sample in all groups.3.Serum albumin and total protein of the different rabbits group at 1st and 2nd sample follow-up Our results revealed that, there was no statistical significance difference between the rabbits groups in albumin at 1st sample; but there were statistical significance differences between them in 2nd sample and in protein at both 1st and 2nd sample. The serum protein levels at 1st sample, thyme group was different from all other groups; but no significant difference was found between other groups. On the other hand, serum protein levels at 2nd sample, the difference was between thyme group and all other groups and between BOL group and control group; but there was no significant difference between ginger group and both BOL or control group. Also there were statistical significantly increase in protein concentration at 2nd sample in all groups compared to 1st sample.4. Serum creatinine levels of the different studied group at 1st and 2nd sample follow-up Our results revealed that, there were statistical significance differences between the studied groups in creatinine in both 1st and 2nd sample. However, there was a significantly decrease in creatinine on the 2nd sample in thyme group compared to 1st sample. Moreover, the difference in 1st sample was between BOL group and all other groups and between ginger group and all other groups; but no difference was found between thyme group and control group. In 2nd sample the difference was between BOL group and all other groups and between thyme group and all other groups; but no difference was found between ginger group and control group. 5.Serum cholesterol and triglyceride of the different rabbits groups at 1st and 2nd sample follow-up The results of our study revealed that, there were statistical significance difference between the rabbits groups in cholesterol and triglyceride in both 1st and 2nd sample. The difference in cholesterol in both 1st and 2nd was between all groups. In triglyceride 1st sample, thyme group was different from all other groups. On the other hand, triglyceride at the 2nd sample was significant difference between thyme group and other groups and between BOL group and all other groups; but no significant difference was found between ginger and control group. However, there were statistical significantly increase in cholesterol in 2nd sample in BOL group and decrease in control, ginger and thyme group compared to 1st sample. Also there were statistical significantly increase in triglyceride in 2nd sample in BOL group and decrease in thyme group compared to 1st sample.6. Serum GSH and IL-6 of the different rabbits groups at 1st and 2nd sample follow-up The results of our study revealed that, there were statistical significance differences between the rabbits groups in GSH in both the 1st and 2nd sample. In GSH 2nd sample, all the groups were different from each others. There was statistical significantly increase in GSH in 2nd sample in control and thyme group and decrease in BOL group compared to 1st sample. The difference in GSH 1st was between BOL group and other groups and between ginger group and thyme group; but no significant difference was found between control group and both ginger and thyme group.The results of our study revealed that, there were statistical significance differences between the rabbits groups in both 1st and 2nd sample. According to the investigation of IL-6 at the 1st and 2nd sample, there was no significant difference found between ginger and thyme group. On the other hand, the difference was between control group and all other groups and between BOL group and all other groups. Statistically, there was a significantly increase in IL-6 at 2nd sample in BOL group and decrease in ginger and thyme group compared to 1st sample. 7.Serum testosterone and growth hormone (GH) of the different rabbits groups at 1st and 2nd sample follow-up The obtained results reveled that, there were statistical significance difference between the rabbits groups in testosterone and GH in both 1st and 2nd samples. The difference between ginger group and all other groups and in all was between BOL group and all other groups; but no difference was found between control group and thyme group. Also there were statistical significantly increase in testosterone and GH in 2nd sample in all groups compared to 1st sample.