الفهرس | Only 14 pages are availabe for public view |
Abstract The present study investigated Vibriosis in cultured Gilthead Seabream at the Suez Canal area, Egypt and tried to develop an effective and inexpensive control method through vaccination. Naturally infected Gilthead Seabream showed severe ascites and hemorrhages with erosions in the skin and musculature of the dorsal region. V. parahaemolyticus, V. alginolyticus and V. fluviales were identified as incriminated pathogens by traditional and molecular (PCR) techniques. Beside, antibiogram profiling of each retrieved Vibrios was also examined. Histopathologically, inflammatory reactions together with degenerative and /or necrotic changes were detected in branchial and visceral tissues. Various developed formalin-inactivated (monovalent & polyvalent) vaccines have experimented; Gilthead seabream groups immunized with each monovalent V. alginolyticus and V. parahaemolyticus bacterins, challenged with the virulent vaccines strains recorded a protection level of 100%, 83.3%; respectively. These values were 91.75%, 75% in fish groups vaccinated with polyvalent FKC of V. parahaemolyticus O11: K40 & V. alginolyticus and V. parahaemolyticus O3: K6 & V. alginolyticus vaccines respectively, which challenged with each virulent corresponding strains. In conclusion, autogenous Vibrios vaccines induced variable protection level, which superior in monovalent FK V. alginolyticus vaccine and polyvalent FKC of V. parahaemolyticus O11: K40 with V. alginolyticus vaccine that could be useful means of prevention and control of vibriosis in cultured Gilthead Seabream in Egypt Key words: Vibriosis, Gilthead Seabream, antibiogram, PCR, histopathology and Vaccines. |