الفهرس | Only 14 pages are availabe for public view |
Abstract Egypt maintains the highest prevalence of HCV infection, affecting an estimated 15%–20% of the population, new cases reported so we search about uncommon source of HCV infection. The present study aimed to detect the occult HCV infection in healthy blood donors from Damietta-Egypt, And detection of HCV core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of HCV RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of HCV core antigen from plasma by ELISA. We founded that: Detection of HCV RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand HCV RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). by comparison between results of HCV core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma. |