الفهرس 
Introduction and RationaleOnly 14 pages are availabe for public view
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Abstract
The present study compares the reno-therapeutic effect of ADSCs, and endogenous stem cell mobilization via G-CSF against Adriamycin-induced chronic nephropathy in rats. The aim of this work is to compare the effect of endogenous CD34+ cells mobilization and ADSCs administration in the treatment of chronic nephropathy in Adriamycin rat model.
A total of 48 adult male albino rats were used. Rats were equally divided into four groups: group I (Control untreated group), group II (Adriamycin challenged group), group III (Adriamycin challenged group and treated with ADSCs) and group IV (Adriamycin challenged group and treated with G-CSF).
The study has shown that both injections of stem cells derived from AT and G-CSF are able to improve Adriamycin-induced chronic nephropathy in rats. Stem cell injections significantly reduced proteinuria and creatinine levels and increase serum albumin associated with Adriamycin-induced nephropathy. The urea level didn’t improve in group III and IV. Both ADSCs and G-CSF injections reduced pathological changes in chronic nephropathy. However, concerning pathological changes, ADSCs treated group showed better improvement than the G-CSF treated group.
Regarding proteinuria, G-CSF treated group showed earlier improvement than ADSCs treated group.
Both ADSCs and G-CSF had an anti-oxidative effect proven by increased TAO and reduced MDA levels in both groups. Also, both ADSCs and G-CSF increase peripheral CD34+ cells percentage, but G-CSF showed a higher percentage.
Homing of ADSCs was detected by using Prussian blue stain to react with iron labeled stem cells in the kidney of group III.
In conclusion, both stimulating endogenous hematopoietic stem cells by G-CSF are at least as effective therapeutic line as injecting exogenous ADSCs in CKD.