الفهرس | Only 14 pages are availabe for public view |
Abstract The killer cell immunoglobulin-like receptors (KIR) control the activation or inhibition of Natural killer (NK) cells by recognition of specific HLA class I ligands, such that target cell cytolysis occur only when activating signals overcome inhibitory signals. The aim of the study is to demonstrate the effect of HLA class I and KIR2DL2 and KIR3DL1 gene expression interaction on the outcome of HCV infection. Material and methods: The study included (33) chronic HCV patient undergone liver transplantation; and (33) adults healthy control. Both the patients and controls are subjected to assessment of (KIR2DL2 and KIR3DL1) gene expression in peripheral blood (PB) and liver tissue by real time polymerase chain reaction( PCR). Moreover, HLA typing of class HLAC for the patient group (33) and for (138) healthy subject as control group were done by sequence specific oliguonucleotides (SSOP) technique utilizing methodology of sequence specific oliguonucleotides probe. results:KIR2DL2 shows significantly high expression in tissue of the patient group compared to the control group with (Pvalue= 0.02),regarding the patient group the KIR2DL2 gene expression in the tissue significantly increase relative to its PB with (P value=0.04).While in the control group no significant difference was observed (P>0.05).On the other hand ,KIR3DL1 gene expression showed no significant difference between the patient and control group, as well as no significant difference was noticed within the patient neither control group(P>0.05). concerning HLA-C typing ,HLA –C1C2 was the most common HLA-C type however no significantly difference between the patients and controls with (P>0.05)conclusion :this study can contribute to the further prospective study with larger scale suggesting the assumption that KIR2DL2 might hampered HCV clearance by inhibiting NK cell killing function affecting both innate and acquired immune response. |