الفهرس 
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Abstract
Brucellosis is a global zoonosis of both public and economic importance caused by the bacteria of the genus Brucella. The aim of this study was to try some modern technologies for control of brucellosis. In the present study Matrix-Assisted Laser Desorption Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF-MS) was evaluated as a tool for Brucella identification that examined Brucella reference strains and local isolates were identified by traditional methods, multiplex PCR and MALDI-TOF-MS. Also pCI-Omp31 was produced to evaluate its efficacy as DNA vaccine in BALB/c mice and recombinant EryC protein was produced to compare its sero-diagnostic efficacy with SBP and HSE as coating antigens for IELISA for accurate diagnosis of bovine brucellosis. It could be concluded that MALDI-TOF-MS strain identification can be achieved with minimal time, labor and cost, making it an attractive alternative to the relatively high investment required for other molecular settings, it demonstrated to be a fast and sensitive tool for identification of Brucella isolates. The use of Omp31 based DNA vaccine as Brucella vaccine for control of brucellosis was found to be effective but it needs more researches on large scale. Also recombinant EryC protein could be a good diagnostic antigen to be used in serological tests for diagnosis of bovine brucellosis and differentiation between infected and Brucella S19-vaccinated cattle.