الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 98 |  |  |
| | | from | 98 | | |
Abstract
Pea microgreens (pisum sativum l.) shoots is a new ready to eat baby leaf vegetable sprouts in Egypt. The internal quality change of baby pea shoots is greatly affected by surrounding environmental condition.
The study was divided into three parts.
Part one: production experiments of green baby pea shoot.
Part two: production experiments of green barley grass.
Part three: production experiments of green baby pea and barley spent sprouts.
The aim of this study was to focus on the impact of predicted climate change conditions (increased CO2 concentration) .growth and yield of baby pea shoot (green sprouts) and barley grass .The main objectives were to evaluate effect of increased CO2 concentration combined with microbial inoculants on photosynthetic pigment (SPAD reading), chemical composition (proximate analysis), energy and mineral content of pea shoot and barley grass and also in spent sprouts.
Part one: production experiments of green baby pea shoot.
For production green pea sprouts rice straw was used as media after sterilized about 15 minutes and then inoculated with three microbial and their combination after soaked pea seed sowing.
The following results were concluded:
(1) Largest numerical yield of baby pea shoot per unit area was recorded in 800ppm CO2 concentration with increasing about 20% more than ambient air followed by CO2 600ppm with increasing about 9.4% than ambient air.
(2) CO2 at 800ppm increased pea sprout crude protein content 37.8%, lipid 46.9% and energy 19.5% per unit area compared with ambient air while decreased carbohydrate content by 5.3%.
(3) combination between Azotobacter chroococcum, Bacillus megaterium, Pseudomonas fluorescens treatment increased significantly pea shoot length, fresh and dry weight /shoot and shoot fresh and dry yield per square meter for both cut numbers (1st and 2nd cut).
(4) Interaction of Az. chroococcum, B. megaterium,Ps. fluorescens with high CO2 concentration 800 ppm recorded the longest shoot and heavier fresh weight of shoot followed by the same bio-fertilizers combination with 600ppm CO2 concentration.
(5) Chlorophyll reading (%) significantly increased with high CO2 concentration 800ppm in both cuts.Also the combination of three bio-fertilizers (Az. chroococcum ,B. megaterium,Ps. fluorescens) recorded the higher chlorophyll reading (%).
(6) Cutting pea sprout shoot 14 days after seed sowing (1st cut) increase all minerals values with medium CO2 concentration (600ppm) followed by lower ambient air concentration while the lowest mineral values of P, K, Ca, Mg, Fe and Zn were recorded in higher CO2 concentration (800ppm).
(7) The combination of three microorganisms (Az. chroococcum ,B. megaterium,Ps. fluorescens) recorded the higher mineral contents in green pea sprout shoot and the lowest mineral values were recorded in non-bio fertilizer treatment (control).Moreover lowest P, Ca, Mg and Zn values was recorded in 800ppm CO2 with non-bio fertilizer (control).
Part two: production experiments of green barley grass.
Sterilized rice straw was used as a media for soaked barley grain sowing and inoculation with the three microbial and their combination.
(1) The interaction between higher CO2 concentrations (800ppm) and biofertilizer(combination of Azotobacter,chroococcum,Bacillus megaterium,Pseudomonas fluorescens) treatments recorded the higher arley shoot length and heavier weight of fresh and dry shoots in both 1st and 2nd cuts.
(2) Barley shoot cut Increased in moisture, protein, lipids ,fiber and ash composition with higher CO2 concentration (800ppm) followed by CO2 at (600ppm), compared with ambient air.
(3) The barley shoot cut protein content increased about 32%in 800ppm CO2 (17.26%) than ambient air (13.08%). However carbohydrate content and energy values decreased significantly with higher CO2 concentration (800ppm) and recorded the higher values in ambient air.
(4) Az. chroococcum combination treatments recorded the higher values of moisture, protein, lipids, fiber and ash content in barley shoot cuts. The higher values were recorded with the three microbes combination (Az. chroococcum ,B. megaterium,Ps. fluorescens).
(5) Single microbial treatment combined with ambient air recorded the higher energy values and decreased with co2 interaction. However no microbe inoculation with ambient (control) recorded the higher energy value.
(6) Marked increase of barley shoot cut (1st cut) in all mineral content (p%, K%, Ca%,Mg%, Fe (ppm) and Zn (ppm) with higher CO2 concentration followed by CO2 at 600 ppm compared with ambient air.
(7) The higher mineral values was recorded in 800ppm CO2 concentration interaction with the three microorganisms (Az. chroococcum ,B. megaterium and Ps. fluorescens) treatment followed by same CO2 concentration with (Az. chroococcum + Ps. fluorescens) treatment. The lowest mineral values were recorded in ambient air with no microbial additive.
Part three: production experiments of green baby pea and barley spent sprouts.
The rice straw media with pea root and seed residues or with barley roots and grain residues in addition to microbial residues named spent sprouts. Yield and analysis results were concluded.
(1) Protein, lipids, and ash in addition to energy, significantly increased with higher CO2 concentration (800ppm) followed by 600ppm CO2 treatment.
(2) The three microorganism combination (Az.chroococcum, B.megaterium, and Ps.fluorescens) recorded the higher values of moisture, protein, lipids, ash and energy in both pea and barley spent sprouts.
(3) Interaction data recorded higher protein, lipids, ash and energy in the interaction between higher CO2 concentration 800 ppm and the three combination microorganisms (Az. chroococcum, B. megaterium,Ps. fluorescens) in both pea and barley spent sprouts.
(4) Moreover the lowest spent sprouts yield was recorded in higher CO2 concentration (800ppm) with the three microorganism combination (Az. chroococcum, B.megaterium, Ps.fluorescens) for both pea and barley spent sprouts.
(5) using CO2 alone or in combination with microorganisms decreased pea spent sprout C/N ratio less than 20 and the lowest C/N ratio results(11.41) was obtained with CO2 at 800ppm with three microorganisms(Az.chroococcum,B.megaterium,Ps.fluorescens)
Combination