الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was conducted to evaluate the efficiency of biologically synthesized platinum nanoparticles (Pt NPs) in treatment of hepatocellular carcinoma compared to cis-platin both in vitro and in vivo. The treatment efficacy of Pt NPs was evaluated by measuring antioxidant activities against oxidative stress caused by diethylnitrosamine (DEN) in liver tissue. The measurements included reduced glutathione (GSH) content and superoxide dismutase (SOD) activity, as well as malonaldialdahyde (MDA) level. Liver function tests were also determined, in addition to the evaluation of serum alpha-fetoprotein (AFP), caspase-3 (Casp-3) and cytochrome c (Cyt c) in liver tissue. Total RNA extraction from liver tissue samples was also done for the relative quantification of B-cell lymphoma 2 (BCL- 2), matrix metallopeptidase 9 (MMP-9) and tumor protein p53 (P53) genes. Histopathological examination was also performed for liver tissue. Results showed that Pt NPs is more potent than cis-platin in treatment of HCC induced by DEN in rats as it ameliorated the investigated parameters towards normal control animals. These findings were well appreciated with hispathological studies shown in DEN group treated with Pt NPs, suggesting that Pt NPs can serve as good therapeutic agents for the treatment of HCC which should attract further studies. |