الفهرس 
Introduction
KidneyOnly 14 pages are availabe for public view
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Abstract
Acute renal injury (ARI) is an abrupt loss of kidney function that develops within 7days. It occurs because of damage to the kidney tissue caused by decreased renal blood flow (renal ischemia) from any cause (e.g. low blood pressure), exposure to substances harmful to the kidney, an inflammatory process in the kidney, or an obstruction of the urinary tract which impedes the flow of urine. ARI is diagnosed on the basis of characteristic laboratory findings, such as elevated blood urea nitrogen and creatinine or inability of the kidneys to produce sufficient amounts of urine.
Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types, including: osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells) but they are recently found to differentiate into endothelial, myocardial, liver, renal and pulmonary epithelial cells. Mesenchymal stem cells have also been shown to have immunomodulatory capabilities and express growth factors known to be renoprotective in experimental acute renal injury (ARI).
This study aims to evaluate the effect of administration of bone marrow derived mesenchymal stem cells in improvement of ischemic / reperfusion induced acute renal injury in adult male albino rat model.
To display the role of MSCs in this model, 60 adult male albino rats were used. Animals were divided into four groups, 15 animals for each group. group I was the control sham-operated group. Three experimental groups were all subjected to I/R injury by clamping both renal pedicles for 40 min. group II was the ischemic group. group III (MSC treated group with local injection) I/R animals received bromodeoxyuridine labeled BM-MSCs locally in renal cortex immediately after removal of the clamps and confirmation of reflow. group IV (MSC treated group with systemic injection) I/R animals that received single intravenous injection of bromdeoxyouridine labeled BM-MSCs in tail vein immediately after removal of the clamp and confirmation of reflow. Animals were sacrificed after 24h, 3 days and 5days of intervention. Kidney was taken out, rapidly fixed, and processed for light microscopic, immunocytochemical, TNFα and IL6 gene expression.