الفهرس | Only 14 pages are availabe for public view |
Abstract This study showed a simple, rapid and economical route to synthesize nano silver using plant extracts. AgNPs size and morphology were characterized using UV-visible analysis at different periods In this respect, total phenolic, total flavonoids and reducing sugars contents were determined in both aqueous and methanolic extracts. Also, FT-IR analysis was conducted to identify the functional groups found in these extracts. On the other hand, zeta potential value was measured in the AgNPs colloids to evaluate the AgNPs stability. AgNPs synthesized with different plant extracts were checked against S. mutans growth. Additionally, cytotoxicity of AgNPs was conducted by MTT assay. The following results have been obtained: 1- 1- Aqueous and methanolic extracts of Ficus microcarpa, M. viridis and Dodonaea viscosa leaves were able to reduce AgNO3 and produce AgNPs with different sizes. 2- 2- AgNPs stability depended on the type of extract and plant species. 3- 3- from TEM analysis, AgNPs formed in the presence of DA (DA AgNPs) had the smallest size (5-19 nm) in comparison with other prepared AgNPs. 4- 4- In general, methanolic extracts were the best in the green synthesis of AgNPs as reductants or capping agents in comparison with aqueous extracts. 5- 6- Chlorhexidine showed the highest antibacterial activity while, all AgNPs had inhibition zones. 6- 7- Cytotoxicity results revealed that it is safe to use AgNPs in the treatment of tooth decay at specific concentrations. |