الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 95 |  |  |
| | | from | 95 | | |
Abstract
Root-knot nematode (Meloidogyne spp.) is a severe soil borne plant
pathogen and causes significant losses in cucumber yield. This study
intended to study isolation and identification of the causal organism
nematode and rhizobacteri from rhizaosphere soil of infested cucumber
with nematode. As well as, study effect of different rhizbacterial isolates
on mortality of M. inconita juveniles (J2) and growth parameter of
cucumber seedlings, In vitro . In addition, study effect of rhizobacterial
isolates and soil amendment on population of M. incognita nematode in
rhizosphere soil and root-tissue of cucumber plants and severity of rootgall,
under greenhouse and field experiments. Also, study effect of
rhizbcterial isolate and soil amendment on growth parameter and the yield
of cucumber plants, under field experiment. Results obtained could be
summarized as follow.
1- Samples of rhizophere soil and infected cucumber plants with rootknot
nematode were collected from Qualubia and Ismailia
governorates for isolation and identification of rhizobacteria and
nematode as a bio-agents organism of root-knot.
2- Collected egg-masses and adult females of nematode were collected
and were identified based on morphological characteristics of the
female perineal patterns. Obtained nematode was identified
Meloidogyne incognita nematode.
3- Collected rhizobacterial isolates were identifed according to
morphological and biochemical characteristic. This identification
was confirmed with Biolog system. These isolates were identifed
as:
a. Bacillus amyloliquefaciens,
b. Rhizobium rhizogenes
c. Serratia maracscens.
53
SUMMARY
Bedour, H. Ahmed (2017), M.Sc., Fac. Agric., Ain Shams Univ.
4- Identified rhizobacterial isolates were tested against M. incognita
juveniles (J2) mortality and the growth parameter of cucumber
seedling, In vitro.
5- Tested rhizobcaterial isolates significantly increased the mortality
of juveniles (J2) nematode compared with the control treatment .The
efficacy of rhizobzcterial isolates were gradually corresponding
with their quantity 106,107 and 108 CFU/ml against juveniles (J2)
nematode. Isolates of B. amyloliquefaciens and R. rhizogenes shown
the greatest activity against juveniles (J2) nemalode than isolate of
S. maracscens, In vitro.
6- Also, In vitro, percentage of seed germination and vigor growth
index of cucumber seedlings were increased with application of
rhizobacterial isolates compared with the control treatment.
Efficacy of rhizobacterial isolates were increased with increasing
their quantity (106,107 and 108 cell/ml). These isolates were the
most effective at 108 cell/ml compared with other bacterial quantity.
There is no difference significant between isolates of rhizobacterial
on seed germination and vigor growth index of cucumber seedlings.
7- In greenhouse experiment, rhizobacterial isolates (B.
anyloliquefaciens, R. rhizogenes and S. maracsens) were applied at
108 cell / ml as soil drench at the same time sowing cucumber
seedling. Also, dry ground leaves of cabbage, eucalypts and garlic
were applied at the rate of 1, 2 and 3g/kg soil as soil treatment 30
days before sowing cucumber seedlings.
8- Rhizobacterial isolates and soil amendments were studied on
population of nematode (M. incognita) in rhizosphere soil
reproduction factors and root tissue developmental stages, females,
egg-masses and egg/egg-masses of cucumber plants and severity of
root- gall nematode.
9- Population of nematode in rhizosphere soil and root tissue of
cucumber plant and severity of rat-gall nematode were reduced with
application of rhizobacterial isolates and soil amendments
54
SUMMARY
Bedour, H. Ahmed (2017), M.Sc., Fac. Agric., Ain Shams Univ.
compared with the control treatment. Population of M. incornita
was significantly decreased after 45 days from planting cucumber
seedlings. Also, the population was slowely increased with
increasing the time after the treatment.
10- Application of soil amendments were more active than
rhizobacterial isolates against population of nematode in
rhizosphere soil and root-tissue of cucumber plants and severity of
root-gall nematode.
11- Isolates of B. amyloliquefaciens and R. rhizogenes were the most
active against population of the nematode in rhizosphere soil and
root-tissue cucumber plants and severity of root-gall nematode
compared with the control treatment, but S. maracsceus isolate was
moderately effective.
12- Dry ground leaves of garlic was the most effective against
population of the nematode in rhizosphere soil and root-tissue of
cucumber plants and severity of root-gall nematode, but dry ground
of cabbage was moderately effective and dry ground leaves of
eucalyptus was less effective compared with the control treatment.
13- Dry ground leaves of garlic as soil amendment and B.
amyloliquefaciens isolate as rhizobacteria were the most effective on
population nematode and severity of root-gall nematode compared
with other treatments, but dry ground leaves of cabbage and
14- In field experiments, rhizobacterial isolates (R. rhizogenes, B.
amyloliquefaciens and S. marascens) were applied at the rate of 108
cell/ml (100 ml/plant) as soil treatment, but soil amendments (dry
ground leaves of cabbage, eucalyptus and garlic) were applied at the
of 3g/palnt as soil treatment. These experiments carried out at
Gezerat El-Dahab region, Giza, where natural heavily infested with
M. incognita nematode.
15- Application of rhizobacterial isolates and soil amendments were
studied on population of M. incognita nematode in rhizosphere soil
55
SUMMARY
Bedour, H. Ahmed (2017), M.Sc., Fac. Agric., Ain Shams Univ.
and in root-tissue of cucumber plants, severity of root-gall
nematode, growth parameter and the yield of cucumber plants.
16- Population of M. incognita in soil and root tissue of cucumber
plants and severity of root-gall was reduced, but growth parameter
and yield of cucumber plants were increased with application of
rhizobacterial isolates and soil amendments as soil treatment
compared with the control treatment. Soil amendments were more
effective than rhizoacterial isolates.
17- Population of M. incognita was significantly reduced after 45
days from treatment with rhizoacter isolates and soil amendments,
but population was slowly increased with increasing the time after
treatment with rhizobacterial isolates and soil amendments.
18- B. amyloliquefaciens and R. rhizogenes isolates as rhizobaterial
were the most effective against population of M. incognita
nematode in rhizosphere and root-tissue of cucumber plants,
severity of rot-gall nematode, growth parameter and yield of
cucumber plant, but isolate of S. maracscens was moderately
effective compared with the control treatment.
19- Dry ground leaves of garlic as soil amendment was the most
effective against population of M. incognita nematode in
rhizosphere soil and root-tissue of cucumber plants, severity of rootgall
nematode, growth parameter and yield of cucumber plants. But,
dry ground leaves of cabbage was moderately effective and dry
ground leaves of eucalyptus was less effective compared with the
control treatment.
20- Application of B. amyloliquefaciens isolate as rhizobacteria and dry
ground leaves of garlic as soil treatment were the most effective, but
R. rhizogenes isolates and dry ground leaves of cabbage were
moderately effective and S. mairucscens isolate and dry ground
leaves of eucalyptus were less effective against population of
nematode, severity of root-gall nematode, growth parameter and
yield of cucumber plants