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Abstract Atotal of 314 samples (82 of each of liver and intestine) collected from diseased broiler in 2013 and(75 of each of liver and intestine) collected from diseased broiler in 2014 were examined bacteriology for the prevelance of C. perfringes . The recorded isolates were subjected to biochemical tests( catalase, sugar fermentation, gelatin liquefaction, litmus milk and indoletests), Nagler`s test, dermonecrotic reactions in albino guinea pigs which proved that all the recorded isolates were C.perfringens types A, D and non toxigenic, toxin antitoxin neutraliation test.Clostridium perfringens was isolated from 132 samples , 91 (66.93%) of them were toxinogenic that subtyped into type A 75 (57.57%) and typeD 15 (11.36%) by intradermal inoculation in G.pig.The higher incidence of C.perfringens was recorded in winter (57.5% in 2013 and 53.12% in 2014) autumn (50% in 2013 and 50% in 2014) then summer (37.93%) in 2013 and 32.60% in 2014) and the lower incidence was recorded in spring (31.25% in 20013 and 30% in 2014). Isolation rate of Clostridium perfringens from intestine and liver samples of diseased chicken in 2013 and 2014 was 65.85% and 58.66% ,respectively from intestine and 21.95 % and 21.33% ,respectively from liver.PCR protocol used for amplification and detection of alpha and epsilon toxin gene of 6 isolates of Clostridium perfringens .profiling of the alpha and epsilon toxin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The most effective antibiotic was Recommended for treatment of NE was applied by sensitivity test to 91 isolates of toxinogenic Clostridium perfringens The histopathological changes of intestine and liver samples of the diseased birds with N.E was disscused in details. |