الفهرس 
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Abstract
This study was carried out at the Laboratory of Physiology and Biotechnology, Animal Production Department, Faculty of Agriculture, Mansoura University, during the period from October 2015 to October 2016. A total of 63 New Zealand White (NZW) mature rabbit does, of about 5 months of age and around 3 kg live body weight (LBW), was used in this study (27 does in the 1st experiment, 12 does in the 2nd experiment and 24 does in the 3rd experiment). Also, 10 fertile bucks, NZW having around 9 months of age and about 3.80 kg average LBW were used for natural mating (1st experiment) and semen collection for achieving in vitro fertilization (2nd and 3rd experiments). I. The first experiment: This study aimed to evaluate the efficacy of daily oral treatment with green tea extract (GTE) at different levels on the reproductive performance, hematology, lipid profile and histological structure of liver and kidney of rabbit does II. The second experiment: Aim of this experiment was to evaluate in vitro maturation, fertilization and development of oocytes recovered from rabbit does treated with GTE as achieved in the 1st experiment (4 does in each group without mating). III. The third experiment (In vitro study): Aim of this study was to evaluate the effect of green tea polyphenols (GTPs) supplementation at different levels in maturation medium of in vitro maturation under normal (35.5°c) and hyper thermal (40.5°c) conditions, and their consequence in vitro fertilization and development of oocytes. The results of the present study could be summarized as follows: THE FIRST EXPERIMENT Results revealed that LBW of rabbit does at the end of treatment, results showed that, in spite the insignificant differences in reproductive performance of does or growth performance of bunnies, results showed that absolute ovarian weight, number of follicles treatment of rabbit does with GTE at a level of 400 mg/kg in G2 and G3 as compared to G1,lower significantly and total), were bleeding(large, LBW (G3) significantly increased WBCs, Hb concentration compared to control (G1) and results showed that treatment of rabbit does with GTE at both levels in G2 and G3 significantly (P<0.05) decreased concentration of total lipids THE SECOND EXPERIMENT Results indicated significant increase in absolute and relative ovarian weight, number of recovered oocyte/doe and recovery rate of oocytes in G2 and G3 as compared to G1, frequency distribution of compact oocytes was significantly higher, while that of denuded oocytes was significantly lower in G2 and G3 as compared to G1, percentage of full expanded oocytes was significantly higher in both treatment groups (G2 and G3) than in G1 (control), being significantly higher in G2 than in G3and the present results revealed that treatment of rabbit does with both levels of GTE showed significant improvement in fertilization rate of in vitro matured oocytes recovered from does in G2 and G3 as compared to G1 (control), being significantly the highest in G2. THE THIRD EXPERIMENT In vitro maturation, in terms of germinal vesicles (GV), germinal vesicles breakdown (GVBD), metaphase-I (M-I), anaphase-I (A-I), metaphase-II (M-II) and degenerated (DG) percentages of rabbit oocytes matured in maturation medium supplemented with GTPs at different levels was studied under normal or hyper thermal conditions, results showed that in vitro maturation of oocytes under hyper thermal condition significantly (P<0.001) decreased percentage of oocytes arrested at M-II stage, while increased those at GVBD, A-I and DG stages as compared to those matured at normal condition. However, percentage of oocytes at GV and M-I stages was not affected significantly by thermal condition and The recorded significant effect of interaction between GTE supplementation and thermal condition on oocytes at M-II stage reflected decreasing M-II oocytes under hyper thermal condition as compared to the normal condition, but effect of GTE level on M-II oocytes was different. Results showed that supplementation of maturation medium with 10 μM/ml GTPs under normal condition yielded significantly the highest percentage of oocytes arrested at M-II stage and the significantly lowest percentage of degenerated. On the other hand, these effects reflected increasing the proportion of oocytes arrested at M-II stage and decreasing degenerated oocytes by increasing GTPs level to 30 μM/ml under hyper thermal condition CONCLUSION It was concluded that daily oral administration of rabbit does 30 days prior to insemination with green tea extract (30% polyphenolic compounds) at levels of 200 mg kg LBW is recommended to improve in vivo reproductive performance and lipid profile of rabbit does without adverse effects on hematological parameters, and liver and kidney functions. Also, treatment of does with green tea extract enhances ovarian activity, oocyte quality, and in vitro embryo production in rabbits. On the other hand, the addition of GTPs as antioxidant to maturation medium under hyper thermal condition seems to improve the in vitro maturation rate of rabbit oocytes and the morula formulation rate.