الفهرس 
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Abstract
The present study was conducted on 364 specimens of Baladi chicks (liver, gallbladder, yolk sac, heart, lung, ceca and spleen from each chick) aged from one to 30 days collected from private farms, Baladi hatcheries and living poultry market in Fayoum Governorate, Egypt. These chicks suffered from respiratory manifestations and associated with diarrhea. Bacteriological examination of samples under test (n=207 and 157) for isolation of Salmonella spp. and E. coli respectively revealed that prevalence were 3.86% (eight isolates) and 14.64% (23 isolate), respectively. Serological identification was shown the predominance of two serotypes of Salmonella spp. (S. Typhimurium (five isolates) and S. Enteritidis (three isolates). On the other hand, serological investigation of the isolated E. coli revealed the circulation of five serogroups and in downward manner they were O157 (eight isolates), O27 (seven isolates), O158 (six isolates), O78 and O153 (one isolate for each). Antimicrobial susceptibility testing revealed that salmonella isolates were sensitive to norfloxacin followed by neomycin while they resisted gentamicin and colistin sulphate. Concerning E. coli isolates, they were sensitive to colistin sulphate and resistant against erythromycin and streptomycin. Finally, PCR applied for detection of antimicrobial resistance genes as tetA, tetB, aac(6)ib-cr. TetA and tetB undetected in tetracycline resistant Salmonella spp. but 50% (five isolates) of the tetracycline resistant E. coli were bearing tetA and tetB. Sequencing of the amplified gyrA gene and their phylogenetic analysis revealed great identity between gyrA of both Salmonella spp. and E. coli. The results concluded the circulation of Salmonella spp. and E. coli not only of zoonotic potential but also bearing transmissible genetic determinate that could transfer intra and inter species.