الفهرس 
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Abstract
Hepatitis C virus )HCV( becomes the endemic virus infection among Egyptian population. The prevalence of the disease is increasing as it ranges from 16-18% of the tested population. The relation between the HCV and the malignancy is not new; However HCV was incriminated in the carcinogenesis of some tumors like hepatocellcarcinoma and bladder cancer. Furthermore, renal cell carcinoma (RCC) is getting more observed in clinical practice with chronic HCV infection. So, the impact of HCV in the tumorgenesis of RCC is hypothesized recently. The mechanism of tumorgenesis and oncogenic influence of HCV with RCC need to be verified. This will be relevant more and more by studying the immunocytopathological characters of HCV virus. To address this aim the following experiments were performed: Collection of 100 renal biopsy and blood samples from patients diagnosed histopathologically as RCC. Qualitative detection of the HCV antibodies in serum using the chemiluminescent microparticleimmunoassay (CMIA) technique. Quantification of HCV-RNA in serum samples that gave positive result in the qualitative detection. Two quantitative methods were used; in house real time PCR technique was used for making a survey for positive HCV samples then a Food and drug administration-in vitro diagnostic (FDA-IVD) approved technique was used for more accuracy. Detection of HCV non structural protein NS3 and NS5A and cell cycle proteins P53 and P21 in infected and non infected tissue samples using immunohistochemistry technique. Detection of the viral like particles in the infected tissue samples by transmission electron microscope. Immunoelectron microscope technique was used for localization of a NS3 and NS5A in infected samples. Western blot technique was used for detection the HCV proteins (NS3 and NS5A) in the infected tissue samples.