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Abstract This study was conducted on fifty apparent healthy adult Japanese quail of both sexes with average age ranged from (8_ 9) weaks and average weight ranged from (250 _ 300gm). After slaughtering of quail, small pieces from cervical and thoracic part of oesophagus, crop, proventriculus and longitudinal section of oesophago _ proventricular junction, proventriculargizzard junction, cranial and caudal sacs of gizzard, middle gizzard and pyloric region were collected. These organs were examined for histological, histochemical, ultrastructural and immunohistochemistry. The oesophageal mucosa was lined by stratified squamous non keratinized epithelium under which the lamina propria contained oesophageal glands. The proventricular mucosa was thrown into plicae and sulci which lined by simple columnar epithelium. This epithelium showed positive reaction to Periodic acid- Schiff (PAS), Alcian blue, Aldehyde fuchsin and Best′s carmine techniques. Compound tubuloalveolar glandular lobules were located in the submucosa. The gizzard had thick middle part, thin cranial and caudal sacs. Simple branched tubular glands opened in gizzard pits. The gizzard glands lined by two type of cells; chief cell and basal cell. While ultrastructural analysis of the cranial sac revealed three types of cells; mucous cells, chief cells and enteroendocrine cells. On the other hand the caudal sac revealed two type of cells; mucous cells and enteroendocrine cells. The Pyloric region characterized by villous appearance of the mucosa. Ultrastructurally, it showed three types of cells; chief cells, mucous cells and enteroendocrine cells. The enteroendocrine cells were identified immunohistochemistry by using antibody against Somatostatin, Gastrin, Glucagon and Serotonin in the stomach. The immunoreactivity of MUC5AC was present in the lining epithelium of mucosal folds of both proventriculus and gizzard and also in the gizzard glands, whereas MUC6 reactivity was found in the oxyntico_peptic cells of proventricular glands. |